After thirty minutes of incubation at +37 1C under an atmosphere containing 5 1% CO2, images of the low face from the inserts were acquired on the Biotek Cytation 5 plate-reading microscope, and analyzed with Halo software (Indica Labs), using the CytoNuclear FL module to count the cells that had crossed the membrane - Current concepts in The identification and characterisation of Rho Kinase Inhibitors

After thirty minutes of incubation at +37 1C under an atmosphere containing 5 1% CO2, images of the low face from the inserts were acquired on the Biotek Cytation 5 plate-reading microscope, and analyzed with Halo software (Indica Labs), using the CytoNuclear FL module to count the cells that had crossed the membrane. Neutrophil activation … Continue reading After thirty minutes of incubation at +37 1C under an atmosphere containing 5 1% CO2, images of the low face from the inserts were acquired on the Biotek Cytation 5 plate-reading microscope, and analyzed with Halo software (Indica Labs), using the CytoNuclear FL module to count the cells that had crossed the membrane